| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043042 |
| Chromosome: | chromosome 10 |
| Location: | 5477546 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458900 | SRR33 | (1 of 39) IPR001190 - SRCR domain; Scavenger receptor cysteine rich (SRCR) protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTGAGGGCGGGCGCCCGCAGCAAGGGC |
| Internal bar code: | GGGCAAAGTCACGGGCCGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 99.7646 |
| LEAP-Seq n confirming: | 6357 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGATCCCGGAAGGTGAG |
| Suggested primer 2: | GCAAGTCAGTCACAGGCTCA |