| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.043065 |
| Chromosome: | chromosome 7 |
| Location: | 2500859 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329550 | VFL5 | Variable flagella protein 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTAATGACCTGCGAACAACACACGCAA |
| Internal bar code: | CCACGGCGTCTGCGATCCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 99.2462 |
| LEAP-Seq n confirming: | 395 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTATATACTGCGGCACCA |
| Suggested primer 2: | CGGCTTTGGAATACGGTAGA |