| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.043083 |
| Chromosome: | chromosome 6 |
| Location: | 8891031 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311000 | FBT2 | (1 of 4) PTHR31585:SF0 - FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC; putative folate/pteridine transporter 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTTGCCTTGGCCGTCAATATATGTGGC |
| Internal bar code: | GGGATCGAGTGTCCCGGGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1309 |
| LEAP-Seq percent confirming: | 99.5723 |
| LEAP-Seq n confirming: | 5122 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATATCAAGCGCTGCAGGT |
| Suggested primer 2: | CGCTCCTGTATCAGAAGGGA |