Insertion junction: LMJ.RY0402.043139_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.043139
Chromosome:chromosome 4
Location:3633089
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g228650 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGATGGGGCCGGGGGCGGGGCGAAGATGA
Internal bar code:TCGTGCGTGCGATTTCATGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:603
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:706
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AATTGGTGGTGTGCTTCTCC
Suggested primer 2:GGAGCAACTTACCCACCAAA