Insertion junction: LMJ.RY0402.043139_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.043139
Chromosome:chromosome 4
Location:3633092
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g228650 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGATCCGGATCCGGACCCGGATCTGGAT
Internal bar code:ATCTCTTGAGGGGGGGGTTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:576
LEAP-Seq percent confirming:97.9756
LEAP-Seq n confirming:22698
LEAP-Seq n nonconfirming:469
LEAP-Seq n unique pos:91

Suggested primers for confirmation by PCR

Suggested primer 1:AATTGGTGGTGTGCTTCTCC
Suggested primer 2:GGAGCAACTTACCCACCAAA