| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.043139 |
| Chromosome: | chromosome 13 |
| Location: | 1122275 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569450 | (1 of 1) IPR000104//IPR029058 - Antifreeze protein, type I // Alpha/Beta hydrolase fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGCTGGTGCTCCCGCCCAGTGCCCGCG |
| Internal bar code: | GATCAGCGTGGGACGGCGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 98.6182 |
| LEAP-Seq n confirming: | 8279 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAGCCGTAGATGCCATA |
| Suggested primer 2: | TTGCCCTTAACAACCACACA |