| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043146 |
| Chromosome: | chromosome 4 |
| Location: | 1716681 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212550 | TTL7 | (1 of 1) K06047 - tubulin---tyrosine ligase [EC:6.3.2.25] (TTL); Tubulin tyrosine ligase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGCGCAGGACTGGTGTTCACCTGAGGC |
| Internal bar code: | AGTTGGAGTCTGCTTCTAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 127 |
| LEAP-Seq percent confirming: | 62.1974 |
| LEAP-Seq n confirming: | 334 |
| LEAP-Seq n nonconfirming: | 203 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGACTTCATGTTGGACGA |
| Suggested primer 2: | CCATTGCAGTACTCTGGGGT |