Insertion junction: LMJ.RY0402.043149_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.043149
Chromosome:chromosome 16
Location:6181326
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g675350 TPP1 Putative tripeptidyl peptidase; (1 of 1) 3.4.14.10 - Tripeptidyl-peptidase II / Tripeptidyl peptidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCATGTCGAACACCCCCGACCCGGGAAT
Internal bar code:TCCCACCACTATTGAAAACCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:303
LEAP-Seq percent confirming:99.7096
LEAP-Seq n confirming:1717
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAAAAGTGCACAAGCGTC
Suggested primer 2:TGCTGACAGGGACTCAAGTG