Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.043160 |
Chromosome: | chromosome 17 |
Location: | 1446135 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g706700 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGGGGATGACGACTGCGGACCCGTGG |
Internal bar code: | CGATCCCGCGCGTCCGGGGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 267 |
LEAP-Seq percent confirming: | 76.9324 |
LEAP-Seq n confirming: | 1901 |
LEAP-Seq n nonconfirming: | 570 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACGCCTAATTCCAATGCAC |
Suggested primer 2: | TCCCATCACGCGTACTAACA |