| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043178 |
| Chromosome: | chromosome 3 |
| Location: | 3964080 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171700 | (1 of 1) 1.2.1.50 - Long-chain-fatty-acyl-CoA reductase / Acyl-CoA reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAAACACACGCACACGCTCCAGGATCGC |
| Internal bar code: | CGACCTTCGGCTTCTCTGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 99.7011 |
| LEAP-Seq n confirming: | 4003 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCCCGCTCTAAATACGA |
| Suggested primer 2: | TAAAGCGAACCAAACCAAGG |