| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043199 |
| Chromosome: | chromosome 7 |
| Location: | 3921934 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339104 | (1 of 2) 3.6.3.19 - Maltose-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACCGCCCAGCAACACACATACACAGTC |
| Internal bar code: | TCTGAGTAGAACAGCTACTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 98.7245 |
| LEAP-Seq n confirming: | 387 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTGGTCATCGCCTAGAG |
| Suggested primer 2: | GCTACAGCCAGTGTGGTTGA |