| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043222 |
| Chromosome: | chromosome 1 |
| Location: | 7836357 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g057791 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGGCAAACTCGACTGTCATATGTAGCA |
| Internal bar code: | GGATTCAAGGGGCGGAAGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 96.7112 |
| LEAP-Seq n confirming: | 941 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCCGCTGTCAGTAACAC |
| Suggested primer 2: | TGCACCCTACGGGACTTTAC |