Insertion junction: LMJ.RY0402.043237_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.043237
Chromosome:chromosome 9
Location:3432002
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g389208 (1 of 2) PTHR11266//PTHR11266:SF17 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // PROTEIN MPV17 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCAACCTTGCACCAAACAACCTGGTGTT
Internal bar code:ACATTCTGGACCGGAGTTGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:174
LEAP-Seq percent confirming:0.996347
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:2981
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAACGGTTTGGTCAAGTG
Suggested primer 2:GACGATGGACGTGTTTGTTG