| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043241 |
| Chromosome: | chromosome 14 |
| Location: | 872964 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613426 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATCAACGGATTCACGTACTCGCGTATTC |
| Internal bar code: | GTAGTTCAGTCTATGGCTAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 91.3295 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTGCAATTGGTATGAAA |
| Suggested primer 2: | GGGCCTTGTTTACATGTGCT |