| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043251 |
| Chromosome: | chromosome 10 |
| Location: | 936100 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424600 | (1 of 1) PF14771 - Domain of unknown function (DUF4476) (DUF4476) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGATGAACAGTCATACGTGGAGCTCGC |
| Internal bar code: | GGATAGACAACGACGTGGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 99.8005 |
| LEAP-Seq n confirming: | 2001 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACTGGTAAGGGTGACGC |
| Suggested primer 2: | ACAACCATCTTCGCAAATCC |