| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043253 |
| Chromosome: | chromosome 9 |
| Location: | 2431845 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391500 | ATG9 | (1 of 1) K17907 - autophagy-related protein 9 (ATG9); Autophagy related | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGGTTGTTGGTCGGGCCGTTGAGACA |
| Internal bar code: | CTACTGGAAGCCGCCTAGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 99.5439 |
| LEAP-Seq n confirming: | 2401 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGTCGCTGTACGAGTC |
| Suggested primer 2: | TCTCGAACCTGCAAAGACCT |