| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.043254 |
| Chromosome: | chromosome 6 |
| Location: | 3670381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278136 | (1 of 1) K15687 - E3 ubiquitin-protein ligase makorin [EC:6.3.2.19] (MKRN) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAGGCAGGCAGTTTGTGAGTGTAGGCG |
| Internal bar code: | ACTGGGGATGGGTCAGTCCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1026 |
| LEAP-Seq percent confirming: | 98.8172 |
| LEAP-Seq n confirming: | 2005 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCTAACATTCCAACACG |
| Suggested primer 2: | CTGGGAGGGGAGGAGTTTAC |