Insertion junction: LMJ.RY0402.043268_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.043268
Chromosome:chromosome 16
Location:1891410
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g655800 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGTGGTACTGGCTGGACTGACGCCTTTG
Internal bar code:GACTTCGCTGTTATATTCGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:821
LEAP-Seq percent confirming:97.1915
LEAP-Seq n confirming:7371
LEAP-Seq n nonconfirming:213
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGAACAAACGACCCACAG
Suggested primer 2:GAGTACATGTTCCTGGCGGT