| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043283 |
| Chromosome: | chromosome 3 |
| Location: | 6376657 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194050 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATGGCCGTATTACCTTTGCGTGTGACC |
| Internal bar code: | TGGTAAAAGACACGGGGTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 99.4614 |
| LEAP-Seq n confirming: | 4432 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGTCCCTTCATCCCT |
| Suggested primer 2: | GGACGTTGGTCCTAGTGCAT |