| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.043291 |
| Chromosome: | chromosome 3 |
| Location: | 2920634 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163050 | (1 of 2) K09955 - hypothetical protein (K09955) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTGGAGGCGCTGAAACCCGTGTGGGCG |
| Internal bar code: | CCTTCCTCAGACTCCTTTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 96.6102 |
| LEAP-Seq n confirming: | 1938 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTATCCACGTCAAGTCAA |
| Suggested primer 2: | GCACGTCCTGGACCATAGAT |