| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043295 |
| Chromosome: | chromosome 16 |
| Location: | 2488500 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660800 | TIM44 | (1 of 1) K17804 - mitochondrial import inner membrane translocase subunit TIM44 (TIM44); Mitochondrial inner membrane translocase subunit | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAAGCGCCAGAGGGACTATAATCCTGTC |
| Internal bar code: | TCCGGATCGATGGCTGCCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 42 |
| LEAP-Seq percent confirming: | 99.3691 |
| LEAP-Seq n confirming: | 945 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGGACATGATGTGGCA |
| Suggested primer 2: | TAATGTAGCAGCGCAGTTGG |