| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.043300 |
| Chromosome: | chromosome 12 |
| Location: | 1688991 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485750 | CGL128 | (1 of 1) PTHR13420//PTHR13420:SF5 - UNCHARACTERIZED // UPF0235 PROTEIN; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACACGATCCGACTGCTTTCCGCCCGCCC |
| Internal bar code: | TGTACCAGTTCACTTGACACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 99.7337 |
| LEAP-Seq n confirming: | 25096 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCGACCTCATCAGCAAG |
| Suggested primer 2: | CAAGCCCTCAAAAATACCCA |