| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043300 |
| Chromosome: | chromosome 12 |
| Location: | 1688993 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485750 | CGL128 | (1 of 1) PTHR13420//PTHR13420:SF5 - UNCHARACTERIZED // UPF0235 PROTEIN; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTCGTTGGCTTCAGGTTGGGGCTGCGG |
| Internal bar code: | GACACTGGGGGTGCGGTTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 98.103 |
| LEAP-Seq n confirming: | 18876 |
| LEAP-Seq n nonconfirming: | 365 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCGACCTCATCAGCAAG |
| Suggested primer 2: | CAAGCCCTCAAAAATACCCA |