Insertion junction: LMJ.RY0402.043307_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.043307
Chromosome:chromosome 7
Location:1441653
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g323150 TEH4 (1 of 1) K17362 - acyl-coenzyme A thioesterase 13 [EC:3.1.2.-] (ACOT13); Thioesterase-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAATGAGAGCACCCGGGCCAGGGCCACC
Internal bar code:CTAATTATCCTCGATCCGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:736
LEAP-Seq percent confirming:99.0066
LEAP-Seq n confirming:897
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AACGGGCTTGAGACTTGAGA
Suggested primer 2:AACTTGGACGACGGTGTAGG