Insertion junction: LMJ.RY0402.043400_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.043400
Chromosome:chromosome 7
Location:5924695
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g354150 MFT22,ARSJ1 Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCACCAGCTTGAACAAACTTGACTGCTT
Internal bar code:TCACCGGGTGCGACACGCGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:219
LEAP-Seq percent confirming:98.6188
LEAP-Seq n confirming:357
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTCCAACACACAAAGCC
Suggested primer 2:AAATACCCCTGACCCCAATC