Insertion junction: LMJ.RY0402.043402_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.043402
Chromosome:chromosome 7
Location:3821005
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g338550 (1 of 1) IPR002048//IPR006585//IPR006652//IPR008979//IPR011043//IPR011050 - EF-hand domain // Fucolectin tachylectin-4 pentraxin-1 // Kelch repeat type 1 // Galactose-binding domain-like // Galactose oxidase/kelch, beta-propeller // Pectin lyase fold/virulence factor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACTCACCCGTAAAAGTCAAGGAACGCCA
Internal bar code:TGGTTTTGGCTCTAAGACTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:469
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGCGACTTCGTGTCAAA
Suggested primer 2:ACAAACATTTAACAGCCGCC