| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043411 |
| Chromosome: | chromosome 1 |
| Location: | 4785880 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033091 | (1 of 1) 6.3.1.14 - Diphthine--ammonia ligase / Diphthamide synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACGCGCAGCGGCGTGTTATAGTATAGG |
| Internal bar code: | CGGCTCTCAAACCTAGAACATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 650 |
| LEAP-Seq percent confirming: | 99.41 |
| LEAP-Seq n confirming: | 337 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTAGCCTTGAGTGACAGC |
| Suggested primer 2: | CGTCGGATCTTGGTTCATTT |