Insertion junction: LMJ.RY0402.043442_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.043442
Chromosome:chromosome 13
Location:2881907
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g583500 TAF2,GEX2 (1 of 5) IPR000104//IPR016024 - Antifreeze protein, type I // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTCATCCGCTGCGGCCCGCCATTTCTGC
Internal bar code:GCACCAGGTCATGAGCGACGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:173
LEAP-Seq percent confirming:99.7963
LEAP-Seq n confirming:980
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGAAGAACAGCGCATGAC
Suggested primer 2:CTGTCGCGAAGGCTCTCTAT