Insertion junction: LMJ.RY0402.043449_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.043449
Chromosome:chromosome 11
Location:1102628
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467678 COP6,HKR2 (1 of 5) IPR001660//IPR013761 - Sterile alpha motif domain // Sterile alpha motif/pointed domain; Histidine cyclase rhodopsin 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGAGCGACCAGGGTGTCGTGACGTGTCG
Internal bar code:GAGGGGCTTAGGGATCGACCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:867
LEAP-Seq percent confirming:95.7975
LEAP-Seq n confirming:26260
LEAP-Seq n nonconfirming:1152
LEAP-Seq n unique pos:182

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGCAAAGAAGGAGGTGAG
Suggested primer 2:CAAGTTGCCAAAGGGTGTTT