Insertion junction: LMJ.RY0402.043451_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.043451
Chromosome:chromosome 3
Location:5155741
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g182200 (1 of 1) IPR001054//IPR028082//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // Periplasmic binding protein-like I // Nucleotide cyclase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAGGGCACTGCACGCCAGCACGACCGAC
Internal bar code:TGGACACTCCCATCGGGGGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:710
LEAP-Seq percent confirming:99.6757
LEAP-Seq n confirming:4611
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTATCATCAGCACGTGGG
Suggested primer 2:TCGCGATCTCAGGTACAGTG