| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043466 |
| Chromosome: | chromosome 5 |
| Location: | 3113305 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239151 | (1 of 3) 6.3.2.25 - Tubulin--tyrosine ligase / TTL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGTTGCTCGCATGCTCGCGCGTGCGTGA |
| Internal bar code: | GGGACAGCCCGGTGCAGTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 99.0115 |
| LEAP-Seq n confirming: | 1202 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACGACTTGCGTGTGCTG |
| Suggested primer 2: | TTTCATTCACACGCAAGCTC |