Insertion junction: LMJ.RY0402.043469_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.043469
Chromosome:chromosome 9
Location:2729621
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g389450 (1 of 1) IPR000104//IPR000504//IPR003613//IPR012677//IPR013083//IPR016024//IPR020683 - Antifreeze protein, type I // RNA recognition motif domain // U box domain // Nucleotide-binding alpha-beta plait domain // Zinc finger, RING/FYVE/PHD-type // Armadillo-type fold // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCGCGGGGGCCGGGGAGGGGTAATGGG
Internal bar code:ACTCGGTTGCTTATTGCAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:417
LEAP-Seq percent confirming:95.769
LEAP-Seq n confirming:1426
LEAP-Seq n nonconfirming:63
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGTCCATAACCTCGCATT
Suggested primer 2:GACAGTCGTTGGGAGGAGAG