| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043477 |
| Chromosome: | chromosome 10 |
| Location: | 2735535 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438400 | LTH2 | LAT2 homolog | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCCTGTGCGACCATGCCCATACCCCT |
| Internal bar code: | GGGGCGACTTGAAGCGAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 70.3094 |
| LEAP-Seq n confirming: | 7000 |
| LEAP-Seq n nonconfirming: | 2956 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTCGTCATGTGTAAGTG |
| Suggested primer 2: | CATGGGCCAACTTCACCTAC |