| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043480 |
| Chromosome: | chromosome 6 |
| Location: | 7267077 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCAAATTTGGTGGGCGCATGCAGGGATT |
| Internal bar code: | CCGAAGTCTGTCGACCTGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 82.1057 |
| LEAP-Seq n confirming: | 5919 |
| LEAP-Seq n nonconfirming: | 1290 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGATGAGCTGAGCGATGT |
| Suggested primer 2: | CAGCCTGAAAATGCAGACAA |