| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043510 |
| Chromosome: | chromosome 17 |
| Location: | 5078231 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734805 | (1 of 8) PF00560 - Leucine Rich Repeat (LRR_1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACGAAACTGCAAAAGCCAAAGCCGGCA |
| Internal bar code: | GTTAGGTGGATTCAGGCGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 98.7228 |
| LEAP-Seq n confirming: | 4406 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGTGATACGTGGGGAAA |
| Suggested primer 2: | AAAAAGGAACATGCCTGCTG |