Insertion junction: LMJ.RY0402.043512_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.043512
Chromosome:chromosome 12
Location:7384295
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g556911 MRE11 Double-strand break repair protein MRE11; (1 of 1) K10865 - double-strand break repair protein MRE11 (MRE11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGGCTTCAGGCAAACACGCACCCCGTGG
Internal bar code:AGCGATTAAGGGTTTCGAGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:45
LEAP-Seq percent confirming:57.7904
LEAP-Seq n confirming:204
LEAP-Seq n nonconfirming:149
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGTTGGGGTGTCAGTCAG
Suggested primer 2:CGTGTGAGTGCGTTTTGTTT