| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043518 |
| Chromosome: | chromosome 6 |
| Location: | 1483907 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259550 | (1 of 2) PTHR23074//PTHR23074:SF20 - AAA ATPASE // GH08677P | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCTGCGCTCTGCCTCCTCCCTCGCGTC |
| Internal bar code: | TACTTGATGATGCTTGGCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 99.0896 |
| LEAP-Seq n confirming: | 3374 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCACTACTCGGTTCGGG |
| Suggested primer 2: | CCCCTCATCATACCAACCAC |