| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043521 |
| Chromosome: | chromosome 11 |
| Location: | 1438699 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467730 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTTGGTGTTGTTATTGCACATTCCCAC |
| Internal bar code: | CCAGCACGGAGAATTCGGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 72.2222 |
| LEAP-Seq n confirming: | 143 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGATGTCTTCGCTTCC |
| Suggested primer 2: | ATTCCGTTAAAGCCCTCGTT |