| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043532 |
| Chromosome: | chromosome 16 |
| Location: | 7173457 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681354 | (1 of 41) PF13920 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGAAAGCACTGGCGTGCTTGGGCGACTA |
| Internal bar code: | CACTAGATTATTGTCTGCCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 99.4608 |
| LEAP-Seq n confirming: | 1660 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGGTGAGTCCTCGTCAT |
| Suggested primer 2: | CAAGTAATAGAGGCCGCTCG |