Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.043562 |
Chromosome: | chromosome_13 |
Location: | 355679 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g564400 | FAP161 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGGCACGCGTTCTGTGCTGTCTGGGAACC |
Internal bar code: | GGGACTGGAATGGGCCTCGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 828 |
LEAP-Seq percent confirming: | 99.8124 |
LEAP-Seq n confirming: | 532 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCCTCTCATCGAAAAAGC |
Suggested primer 2: | CAGAGACACCCATTCCACCT |