| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043565 |
| Chromosome: | chromosome 1 |
| Location: | 3940934 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025700 | (1 of 8) PF00211//PF01547 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_1) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGAACAGCAGGCGGTGTATTTGGACGA |
| Internal bar code: | GGGACAGCTAGCGCTCCAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 717 |
| LEAP-Seq percent confirming: | 86.0703 |
| LEAP-Seq n confirming: | 2107 |
| LEAP-Seq n nonconfirming: | 341 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGAGCCAAGAAAGGTGC |
| Suggested primer 2: | CCAGCATCAACATCAACACC |