| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043585 |
| Chromosome: | chromosome 16 |
| Location: | 1413538 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652250 | CMA3 | Chlamydomonas-specific membrane protein family A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGCGTGTGTGAGGGGCCAGCGGGGGC |
| Internal bar code: | TCATCAAGTGGGCATGAAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 99.4465 |
| LEAP-Seq n confirming: | 1078 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATTATCGACATGTTGCG |
| Suggested primer 2: | ATGCCCTGAACAGGTTTCAC |