| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043585 |
| Chromosome: | chromosome 16 |
| Location: | 1413542 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652250 | CMA3 | Chlamydomonas-specific membrane protein family A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCTCTCGTTCGGCGCCGTGCAACTGCT |
| Internal bar code: | GGTCGGATCGGCTGAATACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 47.3373 |
| LEAP-Seq n confirming: | 240 |
| LEAP-Seq n nonconfirming: | 267 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCCTGAACAGGTTTCAC |
| Suggested primer 2: | GCAATTATCGACATGTTGCG |