| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043620 |
| Chromosome: | chromosome_6 |
| Location: | 5606120 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g285700 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCCAGCCCAGCTCCTCACCCGACTCGGCG |
| Internal bar code: | CATCACGATGGACGTTGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 93.7081 |
| LEAP-Seq n confirming: | 358979 |
| LEAP-Seq n nonconfirming: | 24103 |
| LEAP-Seq n unique pos: | 172 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTAGGTGGTGGATGAG |
| Suggested primer 2: | GGGATTAGTGTCCACACGCT |