| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043620 |
| Chromosome: | chromosome 6 |
| Location: | 5606120 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285700 | (1 of 1) PTHR13466:SF0 - PROTEIN F55C12.5, ISOFORM B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCTTGCGCCAGTCCCATGAGAGAGCAC |
| Internal bar code: | AAAATTAGTCGGGTATGCGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 250 |
| LEAP-Seq percent confirming: | 99.2263 |
| LEAP-Seq n confirming: | 23983 |
| LEAP-Seq n nonconfirming: | 187 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTAGGTGGTGGATGAG |
| Suggested primer 2: | GGGATTAGTGTCCACACGCT |