| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.043650 |
| Chromosome: | chromosome 2 |
| Location: | 3655619 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095151 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACTGGTGAGTGCTCACTATGACAGAGCC |
| Internal bar code: | AGTAGACTTCTGAAGATTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.3079 |
| LEAP-Seq n confirming: | 3300 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTGTTCGACGTATCCT |
| Suggested primer 2: | CTGGACCTAGCTGCATCACA |