| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043655 |
| Chromosome: | chromosome 6 |
| Location: | 5013237 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280200 | (1 of 1) PTHR15141//PTHR15141:SF53 - TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3 // PROTEIN T23E1.3, ISOFORM A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCTAGTCGCTGCAACACCTTCGTCTCC |
| Internal bar code: | TGGGAGTCTTAGGGCGCGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 679 |
| LEAP-Seq percent confirming: | 99.6416 |
| LEAP-Seq n confirming: | 1390 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGATATCATGGACCGT |
| Suggested primer 2: | AGGCGTTAGAGGTTTTGGGT |