| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.043699 |
| Chromosome: | chromosome 7 |
| Location: | 871062 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318651 | AOF4 | (1 of 1) 1.5.3.16 - Spermine oxidase / SMO; Flavin-containing amine oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGCCAGCCACATTACCGCCAGTGGGTG |
| Internal bar code: | GACTCGGTAGCTTCGCGCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 99.7052 |
| LEAP-Seq n confirming: | 7441 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACAGAGGATGCAGTGAA |
| Suggested primer 2: | CCCACCCGATGCATTTATAC |