| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043706 |
| Chromosome: | chromosome 11 |
| Location: | 1297554 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467711 | (1 of 3) PTHR30612 - SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGGGGTTGGGCGGGGAGGGGCAACAT |
| Internal bar code: | TCGGCAAATAGGTCAGGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 14 |
| LEAP-Seq percent confirming: | 81.9672 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGCGTATCAAACCACAC |
| Suggested primer 2: | GACATAGACGCAGACCAGCA |