| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043736 |
| Chromosome: | chromosome 1 |
| Location: | 784922 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004124 | (1 of 3) 3.2.1.8 - Endo-1,4-beta-xylanase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGGAAGAACGTCTCACGGAAGACACG |
| Internal bar code: | TCATTGTTTGGAACCCCGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 779 |
| LEAP-Seq percent confirming: | 99.0042 |
| LEAP-Seq n confirming: | 2386 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCCGTGACGCACAAACAT |
| Suggested primer 2: | CAGTTGTTGGATTGGACACG |